Facts-enriched characterization of cell populations. Morphological details from photographs can incorporate on the richness of flow cytometry information. As an example, the figure reveals an usually standard apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Each individual populace to reveal morphologically distinct attributes. These insights couldn't happen to be received from multiplex staining by itself.

Regardless of whether you’re a starter or a professional, take a look at our resources to progress your exploration and abilities in flow cytometry.

(B) RAW 264.7 macrophages are often annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The quantity of singlets accessible for Investigation boosts after the person trains and employs a more exact product.

Learn new insights into your experimental data by visualizing populations utilizing mixed fluorescence and morphology details. Our software interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment which might be built into your software for an intuitive, streamlined info Examination encounter.

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Moreover, it is illuminating to view how many doublets slip by regular singlet gates, and great to validate the status of unexpected double-positive situations in my Assessment."

Pair your Attune flow cytometer with an autosampler for greater workflow efficiency, and add robotic integration for max throughput.

Consistent graphic good quality even at large throughput Acoustic focusing and also a higher-speed digicam combine to graphic these T cells regularly at very low or large flow charges. Simply regulate target and camera configurations to meet experimental needs.

Early indications of this contamination experienced to begin with been dismissed as particles. click here Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also lets you use morphological options to find exciting subpopulations that could not be apparent from flow cytometry info on your own.

Customers can view event photos in true-time to confirm sample high quality, improve protocols, and file the morphology of every cell population identified for Examination. Imaging capture also Gains from acoustic focusing, which helps to posture the cells in order that a pointy, centered graphic is obtained.

AI-pushed automated impression Examination While using the Attune Cytometric Computer software interprets celebration capabilities into unique morphology parameters which might be coupled with normal fluorescence and scatter parameters to expose hugely reproducible inhabitants alterations and exclusive subsets.

Software Observe: Automatic picture analysis lowers user-to-person variability in flow cytometry gating procedures

Constructed-in dimensionality reduction applications elevated resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to confirm expected population resolution in lysed full human blood. Consumer can view occasions from Every cluster for Visible confirmation of cell phenotypes.

No clean/no lyse leukocyte Evaluation with imaging. Cells have been acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Professional UX/UI enables end users to train AI versions for expanded applications. (A) The “Teach Design” aspect presents annotation tools and real-time design coaching precision.

At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological innovation, which utilizes seem waves to meticulously align cells in the flow cell. This impressive solution surpasses traditional hydrodynamic procedures, resulting in more quickly processing speeds, Extraordinary facts quality, and greater sample throughput.

As the mobile line had Beforehand been analyzed about the Attune CytPix Flow Cytometer, the scientists went again to the pictures and had been capable to document the microbial an infection a minimum of 5 days earlier.

Agilent Worth Assure ensures you at the least 10 years of instrument use in the date you buy. Or else, We're going to credit you With all the residual value of the process towards an upgraded product.

Right here, we display that by gating tactics using graphic-primarily based quantitative parameters can distinguish interacting cells from coincident events to much more correctly analyze interacting cells.