Knowledge-enriched characterization of mobile populations. Morphological information and facts from visuals can add towards the richness of flow cytometry details. One example is, the determine reveals an normally regular apoptosis assay working with Annexin V and PI, incorporating cell imaging to characterize cells in Every population to expose morphologically distinctive options. These insights could not have been obtained from multiplex staining on your own.

Whether you’re a novice or an authority, investigate our assets to progress your investigate and skills in flow cytometry.

Combine acoustic focusing engineering with higher-parameter Assessment for flexible spectral unmixing and conventional workflows.

The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a significant-pace brightfield digital camera for simultaneous substantial throughput flow cytometry and high resolution brightfield imaging.

"A picture is just not rather worth one thousand dots, nonetheless it actually helps you to see illustrations or photos. It is excellent to determine visuals that correlate with staining. Such as, AnnexinV-favourable cells that are morphologically distinct than healthy cells, or CD14+ monocytes which can be larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.

On top of that, it can be illuminating to discover how many doublets slip by means of standard singlet gates, and wonderful to validate the standing of surprising double-favourable events in my Examination."

Optimizing gating strategy. Even sturdy manual singlet gating is mistake-susceptible and continues to be a subjective choice stage in Just about all flow cytometry assays. Imaging can be used to confirm and change gates to incorporate only single cells of curiosity.

Consistent image excellent even at significant throughput Acoustic focusing and a high-pace digicam Merge to image these T cells continually at very low or higher flow fees. Quickly modify aim and camera configurations to fulfill experimental prerequisites.

The Attune NxT cytometer could be the responsible workhorse instrument your lab can depend on for steady and trusted overall performance. The Attune CytPix model includes a higher-velocity brightfield digicam and AI-driven impression Examination software, with the latest updates which include device Studying instruments and an intuitive user interface, enabling enhanced info precision, precision, and speedier insights into sample biology.

Maybe most of all, these activities contain cells of Plainly diverse phenotypes which could lead on to incorrect conclusions regarding double positive occasions (specifically in exceptional populations).

Audio waves empower specific mobile alignment, resulting in Fantastic knowledge excellent and ~10X speedier sample flow prices.

Software Be aware: Automatic graphic Investigation lessens user-to-consumer variability in flow cytometry gating tactics

Leverage the power of Highly developed technologies and innovation to accelerate your discoveries. Belief inside the Attune Flow Cytometer relatives to deliver the dependability, speed, and precision that established your operate aside.

No clean/no lyse leukocyte Evaluation with imaging. Cells had been acquired from 24-hour-aged blood by dilution in one mM EDTA ( Skilled UX/UI allows buyers to train AI styles for expanded applications. (A) The “Coach Product” attribute presents annotation applications and authentic-time model coaching precision.

Gating on these prolonged parameters helps you to rapidly and correctly discover populations of fascination and ensure gating technique with little if any guide evaluate. The Attune program offers use of point out-of-the-artwork machine Studying Assessment resources within just an expertly intended consumer interface, making it exceptionally simple to use.

Because the cell line had previously been analyzed over the Attune CytPix Flow Cytometer, more info the scientists went back to the pictures and have been in the position to doc the microbial infection at the least 5 days previously.

Agilent Benefit Promise assures you at the very least a decade of instrument use through the day you buy. In any other case, We are going to credit score you While using the residual worth of the program toward an upgraded model.

Listed here, we display that by gating strategies using graphic-centered quantitative parameters can distinguish interacting cells from coincident situations to a lot more properly examine interacting cells.