Information-enriched characterization of mobile populations. Morphological information from photos can insert for the richness of flow cytometry data. As an example, the figure displays an or else regular apoptosis assay working with Annexin V and PI, incorporating mobile imaging to characterize cells in each inhabitants to expose morphologically distinctive options. These insights could not have already been attained from multiplex staining by yourself.

Whether or not you’re a newbie or an expert, explore our sources to advance your exploration and knowledge in flow cytometry.

(B) RAW 264.7 macrophages tend to be annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The number of singlets obtainable for analysis will increase when the person trains and employs a far more exact product.

Uncover new insights into your experimental details by visualizing populations using blended fluorescence and morphology facts. Our software package interface wizards help downsampling, concatenation (merged data files) and dimensionality equipment which are developed into your computer software for an intuitive, streamlined details Examination experience.

"A picture is not really truly worth one thousand dots, but it definitely helps you to see photos. It is great to discover illustrations or photos that correlate with staining. For example, AnnexinV-good cells which are morphologically different than healthful cells, or CD14+ monocytes that are more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.

Also, it is actually illuminating to determine what number of doublets slip by classic singlet gates, and fantastic to verify the standing of sudden double-optimistic situations in my Assessment."

Pair your Attune flow cytometer with an autosampler for amplified workflow effectiveness, and add robotic integration for max throughput.

Reliable impression quality even at significant throughput Acoustic focusing in addition to a significant-pace camera Merge to impression these T cells constantly at very low or significant flow costs. Conveniently regulate emphasis and digicam configurations to fulfill experimental specifications.

The Attune NxT cytometer would be the responsible workhorse instrument your lab can depend upon for dependable and dependable functionality. The Attune CytPix product includes a large-velocity brightfield digital camera and AI-driven image Investigation computer software, with recent updates like equipment Mastering tools and an intuitive consumer interface, enabling Improved details precision, precision, and faster insights into sample biology.

Consumers can check out function photographs in actual-time to substantiate sample good quality, improve protocols, and history the morphology of every cell populace identified for Examination. Imaging seize also Gains from acoustic focusing, which helps to placement the cells so that a sharp, centered image is acquired.

Seem waves enable exact mobile alignment, resulting in Excellent facts quality and ~10X faster sample flow charges.

We Formerly demonstrated the strength of imaging Auto-T/Ramos mobile interactions. Allow’s take a look at just the inhabitants of best fascination, the double beneficial situations, to learn more. We can now use extended morphological parameters (in this article, circularity and skewness of intensity) to even more look at the characteristics of these populations and refine gating on these activities, rising info robustness.

Constructed-in dimensionality reduction instruments enhanced resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify predicted populace resolution in lysed entire human blood. User can perspective events from Each individual cluster for visual confirmation of mobile phenotypes.

Flexible payment program from Agilent Financial Remedies help you receive the vital instrumentation you will need for quick task, or to improve your lab.

At the center of every Attune Flow Cytometer lies our revolutionary acoustic concentrating technologies, which utilizes seem waves to meticulously align cells in the flow cell. This progressive tactic surpasses traditional hydrodynamic procedures, resulting in a lot quicker processing speeds, exceptional website facts quality, and improved sample throughput.

The Attune Xenith cytometer is a significant-parameter spectral analyzer, supplying equally spectral unmixing and conventional flow capabilities for detailed and flexible mobile Investigation.

Agilent Benefit Promise guarantees you a minimum of a decade of instrument use through the date you buy. Or else, We'll credit you Along with the residual price of the technique towards an upgraded design.

In this article, we exhibit that by gating tactics utilizing picture-primarily based quantitative parameters can distinguish interacting cells from coincident occasions to a lot more accurately assess interacting cells.